Biosynthesis of 2-methylisoborneol is regulated by chromatic acclimation of Pseudanabaena
Ming Su1,2,7,*, Jiao Fang1,8, Zeyu Jia1,3, Yuliang Su4, Yiping Zhu5, Bin Wu4, John C. Little6, Jianwei Yu1,2,7, Min Yang1,2,7
1. Key Laboratory of Drinking Water Science and Technology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, P.O. Box 2871, Beijing, 100085.
2. National Engineering Research Center of Industrial Wastewater Detoxication and Resource Recovery, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, P.O. Box 2871, Beijing, 100085.
3. Yangtze Eco-Environment Engineering Research Center, China Three Gorges Corporation, Beijing, 100038.
4. Zhuhai Water Environment Holdings Group Ltd., Zhuhai, 519020.
5. Shanghai Chengtou Raw Water Co. Ltd., Beiai Rd. 1540, Shanghai, 200125.
6. Department of Civil and Environmental Engineering, Virginia Tech., Blacksburg, VA, 24061-0246.
7. University of Chinese Academy of Sciences, Beijing, 100049.
8. School of Civil Engineering, Chang’an University, Xi’an, 710054.
* Corresponding Author: Ming Su (mingsu@rcees.ac.cn)
Supplementarty Material
Figures and/or tables are provided below as the supplemenatary evidences to the main text.
Images of culture solution
Relative abundances of pigments under different light color culture conditions
Four typical cyanobacterial photosynthetic pigments were identified according to their fluorescence excitation (Table 1).
| Photosynthetic pigments | excitation wavelength | emission wavelength |
|---|---|---|
| Chlorophyll a (Chla) | 427 nm | 680 nm |
| phycoerythrin (PE) | 488 nm | 588 nm |
| phycocyanin (PC) | 600 nm | 660 nm |
| Allophycocyanin (APC) | 633 nm | 660 nm |
| Carotenoids | 513 nm | 556 nm |
The relative abundances of each pigment were further estimated by the absorption spectrum of the culture solution under different light color conditions. In more detail, Chl a, PC, PE and carotenoids were identified and APC was not detected in the culture solutions according to Table 1, hence we regarded the amount of APC is ignorable. Subsequently, we identified the absorption spectrums of each pure pigment (Fig. 3), and linear models were constructed between the absorption spectrum of culture soloutions (\(Y_i\), \(i = (W, R, G, B)\)) and Chl a (\(p_1\)), PC (\(p_2\)), PE (\(p_3\)) and carotenoids (\(p_4\)) for different light color culture conditions Eq. 1, and the relative abundances of each pigments of culture solutions under white (\(\lambda_W\)), Red (\(\lambda_R\)), Green (\(\lambda_G\)), Blue (\(\lambda_B\)) light conditions were determined according to the least squares algorithm.
\[ Y_i=\lambda_{1i}p_1+\lambda_{2i}p_2+\lambda_{3i}p_3+\lambda_{4i}p_4 \tag{1}\]
